จารุวรรณ (หยก) จรรอด
Sales and Product Specialist
0628699284
jaruwan@expedeon.com
RunBlue Gel Tank
Brand : Expedeon
เครื่องแยกสารโปรตีนด้วยกระแสไฟฟ้าในแนวตั้ง และส่งถ่ายโปรตีนจากเจลสู่เมมเบรน
Last Update : 09:46:10 05/09/2017
FASP Protein Digestion Kit
Brand : Expedeon
ชุดย่อยโปรตีนตัวอย่างเพื่อวิเคราะห์ Mass spectrometry
Last Update : 09:45:31 05/09/2017
สารแบรดฟอร์ด - Bradford Ultra
Brand : Expedeon
สารวัดปริมาณโปรตีน Bradford แบบพร้อมใช้ (ready-to-use)
Last Update : 09:45:13 05/09/2017
Amintra Glutathione Resin
Brand : Expedeon
Affinity resin สำหรับแยก GST-tagged protein ให้บริสุทธิ์
Last Update : 09:44:54 05/09/2017
Prestained Marker: Tri-colour
Brand : Expedeon
สารเทียบขนาดโปรตีนมาตรฐาน สามารถใช้วิเคราะห์โปรตีนที่มีขนาด 5 kDa - 180 kDa
Last Update : 09:44:18 05/09/2017

Page View  63  
Product Information :

Product Name:  
สารแบรดฟอร์ด - Bradford Ultra


Product Category:   เครื่องมือวิทยาศาสตร์ / สารเคมีและวัสดุภัณฑ์สำหรับห้องปฏิบัติการ
Product Sub-Category:   การหาปริมาณโปรตีน
Brand:   Expedeon 
Product Type :   สินค้า, ผลิตภัณฑ์ 
Short Description :   สารวัดปริมาณโปรตีน Bradford แบบพร้อมใช้ (ready-to-use)
     
 

Expedeon's  BradfordUltra is a quick and ready-to-use Coomassie-binding, colorimetric method for total protein quantitation in an environment containing up to 1% detergent (1% high protein range, 0.1% low protein range).

 

BradfordUltra

Detergent compatible protein quantitation

When Coomassie dye binds protein in an acidic medium, an immediate shift in absorption maximum occurs from 465 nm to 595 nm with a concomitant color change from brown to blue. Protein concentrations are estimated by reference to absorbances obtained for a series of standard protein dilutions, which are assayed alongside the samples with unknown protein concentrations.

 

Expedeon's BradfordUltra is an improvement over classical Bradford formulations which cannot tolerate detergent contamination of the protein samples. In addition, the BradfordUltra reagent shows excellent linearity for a defined range of protein concentrations and shows significantly less protein-to-protein variation than is observed with other Bradford-type Coomassie formulations.

Detergent Compatibility

Comparison of Expedeon's BradfordUltra Assay with Pierce's "Coomassie Protein Assay. The graph shows the average blank corrected A595 measurements for detergents samples (no protein). When detergents are present in the sample, competitor Bradford assay solutions give high blank readings making accurate detection of the protein sample impossible. BradfordUltra is not affected by the detergents compared to Classic formulations and provides excellent signal to noise ratios and accurate determintation of the protein concentration regardless of the detergent presence.

 Bradford Assay Signal

 

Pierce Bradford Assay

 

Expedeon BradfordUltra Assay

 

Standard curves obtained with BradfordUltra are unaffected by the presence of detergents.

Standard curves obtained with classical Bradford formulation are significantly affected by thepresence of detergents resulting in loss of sensitivity and inaccurate results.

 

Protocol

1) Make a dilution series of the chosen model protein in the range:

0.1 mg/ml - 1.5 mg/ml (high protein range) OR 

1 g/ml - 25 g/ml (low protein range)

 

2) Mix the samples, standards and a blank (buffer, no protein) with BradfordUltra reagent.

High Protein Range: 1 part sample for 15 parts reagent

Low Protein Range: 1 part sample for 1 part reagent

 

3) Read absorbance at 595 nm.

4) Calculate concentration:

Subtract the average 595 nm measurement for the blank from the 595 nm measurements of all other individual standards and unknown samples. Plot the average blank-corrected 595 nm measurement for each standard vs. concentration. Use the slope of this standard curve to estimate the protein concentration of the unknown samples.

 

Product Related
BradfordMX
Brand : Expedeon
สารวัดปริมาณโปรตีน Bradford แบบพร้อมใช้ (ready-to-use)
© 2000-2008 CopyRight by Expedeon Asia Pte. Ltd.
Tel. 099-495-1684  Fax. 02-834-1684  Website. www.expedeon.com
disclaimer | privacy | contact us 

  Sale Login Warehouse Login Driver Login