Affinity resins have been widely used for the purification of enzymes and other proteins that bind nucleotides and related molecules. ATP-agarose resin comprises ATP attached to agarose beads via its gamma-phosphate. A long hydrophilic spacer (14-atom) is used to minimize unwanted hydrophobic interactions and to facilitate unhindered interactions with biomolecules. The ligand is coupled through the gamma-phosphate group which means that the resin is resistant to phosphatases found in many crude tissue extracts.
Quick and easy-to-use
Can be used in column chromatography or batch processing