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Product Information :

Product Name:  
Automated Protein Digestion & MS Sample Preparation

Product Category:   เครื่องมือวิทยาศาสตร์ / สารเคมีและวัสดุภัณฑ์สำหรับห้องปฏิบัติการ
Product Sub-Category:   Life Science
Brand:   CEM 
Product Type :   สินค้า, ผลิตภัณฑ์ 
Short Description :   The DigestPro? is a dedicated protein digestion and sample preparation system for MS based proteomics.
     
 

Overview


The DigestPro™ is a dedicated protein digestion and sample preparation system for MS based proteomics. It completely automates all essential steps of protein digestion protocols and easily works with both in-solution and in-gel based methods.

In addition sample clean-up and MALDI spotting can be automated. It achieves high throughput with up to 96 samples at a time. The whole procedure is performed under controlled and reproducible conditions that is essential for protein analysis by mass spectrometry.

 

Features

  • Automated in-gel or in-solution digestion
  • Ready-to-run optimized programs with full customizability
  • Freely selectable temperature for each step (heating and cooling)
  • Use of standard lab buffers and solutions (low running costs)
  • Desalting and concentration with ZipTips (or similar reversed phase tips)
  • MALDI target spotting
  • Modular work area setup for individual instrument configuration
 
 

Simplified Proteomics
Easily prepare samples for protein analysis by mass spectrometry.


The DigestPro is a single instrument for in-solution digestion, in-gel digestion, and sample clean-up including desalting and enrichment.
Ready-to-use protocols can be immediately used for high-throughput rapid automation of all steps necessary. Additionally, all steps can be easily customized as needed.

Solution Digestion

  1. Reduction of disufide bonds
  2. Alkylation of cysteine residues
  3. Enzymatic Digestion (up to 70°C)
  4. Clean Up and Enrichment with Zip Tips or StageTips

In-Gel Digestion

  1. Reduction of disulfide bonds
  2. Alkylation of cysteine residues
  3. Enzymatic digestion (up to 70°C)
  4. Optional: Clean-up and Enrichment with ZipTips or StageTips
  5. Washing of gel slices and elution of peptides. The needle adds liquids from a distance, never touching the sample and thus avoiding any risk of cross contamination

 

 
 

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