Automated In Situ Hybridization and Immunohistochemistry System

InsituPro™

ISH and IHC Automation for Whole Mounts and Slides

Overview

The InsituPro is a dedicated system for automated in situ hybridization (ISH) to prepare samples for detection of DNA or RNA(mRNA, miRNA) and also for immunohistochemistry (IHC) to detect proteins and other antigens. The system automates all of the tedious and repetitive washing and incubation steps for ISH and IHC processes.

Features

Use Whole Mounts and Slides

The InsituPro easily accommodates both slides and whole mount samples. Easily switch between samples in only minutes. Up to 60 whole mounts/vibratome sections or slides can be processed in parallel with individual probes or antibodies.

Slides with thin sections are mounted on a counter slide forming an independent incubation chamber. Six of these chambers are combined to a slide block and ten of these blocks are mounted vertically into the heated incubation tub. The incubation tub allows for control of temperature and high humidity for optimal performance. Liquid exchange is driven by gravity flow and capillary forces ensure the sections are submerged in buffer.

Widely Used

The InsituPro has a strong record for In situ Hybridization and Immunohistochemistry.

• Used at > 300 research groups worldwide
• Cited in > 550 publications
• Applicable to zebrafish, mouse, Ababidopsis, Drosophila, Xenopus, chicken, and more

Easily Adaptable Protocols

The InsituPro comes with ready to use protocols for a wide range of species. However, each protocol is easily adaptable and allows full control of all parameters including temperature settings and parallel processing of samples with different incubation times. Individual treatment of each samples is also possible including the use of unique antibodies and probes.