Amintra Desalting Spin Column
Cross-linked neutral particles with very small pores are packed into spin tubes for rapid desalting and / or buffer exchange. Amintra Desalting Spin Columns achieve desalting or buffer exchange in just a few minutes. Up to 24 samples (depending on the type of microcentrifuge) of 10 – 100 µl can be processed in one spin. It is a much faster and more efficient approach when compared to dialysis tubes or membrane ultrafiltration.
Key benefits:
• Rapid desalting or buffer exchange
• Very little loss of target molecules (typically > 95% recovery)
• Suitable for proteins (> 6,000 dalton) and DNAs (> 10 bp)
• DNAse free
Amintra Desalting Spin Column is particularly useful for the following applications:
• Desalting of histidine-tagged proteins (e.g. imidazole and NaCl) recovered from IMAC Amintra Desalting Spin Colum
• Desalting of samples before loading to SDS-PAGE or GELFREE 8100
• Desalting of samples before conducting other analysis
• Buffer exchange, for example, after low pH elution
• Desalting of DNAs The small porous particles provide huge surface area with very short diffusion distance, which means small molecules such as salt can be partitioned rapidly. In comparison, both dialysis tube and membrane ultrafiltration have very low surface areas. It always takes much longer to conduct dialysis (typically from a few hours to a few days). Membrane ultrafiltration of small samples always experiences membrane blockage and severe loss of valuable materials
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